![]() 16501, anti-Gram-negative endotoxin (IVEC) Cat. Monoclonal antibodies used in the study were supplied by QED Bioscience, San Diego, and include anti-quinidine (QD) Cat. The QED SuperMedium formulation was prepared as described by Weng and Chua ( 11). Serum-based formulation changes are increasingly unsuitable for production of human therapeutics, for which animal-free or protein-free, chemically defined cell culture media are preferred or required ( 6).Īll commercial cell culture media were purchased from Sigma-Aldrich. The first chemically defined media approaches showed varying degrees of success, but room for improvement remains ( 3, 4, 5). Another strategy is hyperstimulation by osmolarity stress. Early additions included mouse peritoneal factors, growth inhibitors, autocrine factors, and cyclic nucleotides. Alternative strategies have been used to increase protein output, both before and after the bioprocess trend toward serum-free media. Improving cell culture media has been and continues to be an area of productive research. KEYWORDS: REAGENTS, SERUM, FLASK CULTURE, ROLLER BOTTLES, HYBRIDOMAS, FED-BATCH, BATCH CULTURE WHO SHOULD READ: PROCESS DEVELOPMENT, MANUFACTURING, ANALYTICAL PRODUCT FOCUS: DIAGNOSTIC AND REAGENT ANTIBODIES, THERAPEUTICS ![]() ![]() This process has been implemented for many cell types including hybridomas and Chinese hamster ovary (CHO) cell cultures ( 1, 2). ![]() The fed-batch strategy is typically used in bioindustrial processes to achieve high cell densities in bioreactors and extend the production phase of cultures. The modified “fed-batch” process was developed based on feeding cultures with a growth-limiting nutrient substrate to improve their longevity and cell health. Bioreactors can operate in batch, perfusion, and continuous modes. ![]()
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